Reasonable no clone torrent9/26/2023 The GC-rich regions prone to low coverage include a number of human promoters, so we therefore catalog 1,000 that were exceptionally resistant to sequencing. Pacific Biosciences coverage levels are the least biased, followed by Illumina, although all technologies exhibit error-rate biases in high- and low-GC regions and at long homopolymer runs. As in previous work, library construction conditions significantly influence sequencing bias. We applied these methods to the Illumina, Ion Torrent, Pacific Biosciences and Complete Genomics sequencing platforms, using data from human and from a set of microbes with diverse base compositions. Accordingly, we have developed computational methods for discovering, describing and measuring bias. These biases impair scientific and medical applications. DNA sequencing technologies deviate from the ideal uniform distribution of reads.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |